Anti-Fibrillarin/FBL Antibody Picoband™

Product Name

Anti-Fibrillarin/FBL Antibody Picoband™

View all Fibrillarin Antibodies

SKU/Catalog Number

A03178-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fibrillarin/FBL Antibody Picoband™ catalog # A03178-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Fibrillarin/FBL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03178-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Fibrillarin/FBL recombinant protein (Position: K77-N138).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03178-1 is reactive to FBL in Human, Monkey

Applications

A03178-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

33.784kDa

Background of Fibrillarin

rRNA 2'-O-methyltransferase fibrillarin is an enzyme that in humans is encoded by the FBL gene. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Fibrillarin/FBL using anti-Fibrillarin/FBL antibody (A03178-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillarin/FBL antigen affinity purified polyclonal antibody (Catalog # A03178-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillarin/FBL at approximately 37 kDa. The expected band size for Fibrillarin/FBL is at 37 kDa.

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Figure 2. IF analysis of Fibrillarin/FBL using anti-Fibrillarin/FBL antibody (A03178-1).
Fibrillarin/FBL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Fibrillarin/FBL Antibody (A03178-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U20S cells using anti-Fibrillarin/FBL antibody (A03178-1).
Overlay histogram showing U20S cells stained with A03178-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibrillarin/FBL Antibody (A03178-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For FBL (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1,34-kD nucleolar scleroderma antigen; EC 2.1.1.-; EC 2.1.1.37; FIB; FIB1,34 kDa nucleolar scleroderma antigen; fibrillarin; FLRNrRNA 2'-O-methyltransferase fibrillarin; RNA, U3 small nucleolar interacting protein 1; RNU3IP1 FBL FIB, FLRN, Nop1, RNU3IP1 fibrillarin rRNA 2-O-methyltransferase fibrillarin|34 kDa nucleolar scleroderma antigen|34-kD nucleolar scleroderma antigen|RNA, U3 small nucleolar interacting protein 1|U6 snRNA 2-O-methyltransferase fibrillarin|histone-glutamine methyltransferase

For more info on FBL, check out the FBL Infographic

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In this infographic, you will see the following information for FBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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